@inbook {192,
	title = {Third Generation DNA Sequencing with a Nanopore},
	booktitle = {Nanopores: Sensing fundamental biological interactions at the single molecule level},
	year = {2011},
	pages = {287-311},
	publisher = {Springer US},
	organization = {Springer US},
	chapter = {12},
	abstract = {<p>With the advent of Next-Generation-Sequencing (NGS) technologies, an enormous volume of DNA sequencing data can be generated at low cost, placing genomic science within the grasp of everyday medicine. However, mired in this voluminous data, a new problem has emerged: the assembly of the genome from the short reads. In this chapter we examine the prospects for sequencing DNA using a synthetic nanopore. Nanopore sequencing has the potential for very long reads, reducing the computational burden posed by alignment and genome assembly, while at the same time eliminating logistically challenging and error-prone amplification and library formation due to its exquisite single molecule sensitivity. On the other hand, long high fidelity reads demand stringent control over both the DNA configuration in the pore and the translocation kinetics. We examine the prospects for satisfying these specifications with a synthetic nanopore.</p>
},
	keywords = {Comparison between biological and synthetic nanopores, Hydrodynamic focusing, Molecular dynamics simulations, Sequencing DNA, Trapping DNA, λ-DNA},
	isbn = {978-1-4419-8251-3},
	doi = {10.1007/978-1-4419-8252-0_12},
	url = {http://dx.doi.org/10.1007/978-1-4419-8252-0_12},
	author = {Gregory Timp and Mirsaidov, Utkur and Timp, Winston and Shim, Jiwook and Wang, Deqiang and Valentin Dimitrov and Scrimgeour, Jan and Lin, Chunchen and Jeffrey Comer and Anthony H. Ho and Zou, Xueqing and Aleksei Aksimentiev and Klaus Schulten},
	editor = {Iqbal, Samir M. and Rashid Bashir}
}
