@article {286,
	title = {A synthetic enzyme built from DNA flips 10$^{7}$ lipids per second in biological membranes},
	journal = {Nature Communications},
	volume = {9},
	year = {2018},
	month = {07/2018},
	pages = {2426},
	abstract = {<p>Mimicking enzyme function and increasing performance of naturally evolved proteins is one of the most challenging and intriguing aims of nanoscience. Here, we employ DNA nanotechnology to design a synthetic enzyme that substantially outperforms its biological archetypes. Consisting of only eight strands, our DNA nanostructure spontaneously inserts into biological membranes by forming a toroidal pore that connects the membrane{\textquoteright}s inner and outer leaflets. The membrane insertion catalyzes spontaneous transport of lipid molecules between the bilayer leaflets, rapidly equilibrating the lipid composition. Through a combination of microscopic simulations and fluorescence microscopy we find the lipid transport rate catalyzed by the DNA nanostructure exceeds 10<sup>7</sup> molecules per second, which is three orders of magnitude higher than the rate of lipid transport catalyzed by biological enzymes. Furthermore, we show that our DNA-based enzyme can control the composition of human cell membranes, which opens new avenues for applications of membrane-interacting DNA systems in medicine.</p>
},
	keywords = {Computational biophysics, DNA nanostructures, Membrane biophysics, Nanobiotechnology},
	doi = {10.1038/s41467-018-04821-5},
	url = {http://www.nature.com/articles/s41467-018-04821-5},
	author = {Ohmann, Alexander and Li, Chen-Yu and Maffeo, Christopher and Al Nahas, Kareem and Baumann, Kevin N. and G{\"o}pfrich, Kerstin and Yoo, Jejoong and Keyser, Ulrich F. and Aleksei Aksimentiev}
}
